Bovine Sterol Regulatory Element Binding Proteins ELISA kit (E11R0363)

BlueGene Biotech Bovine Sterol Regulatory Element Binding Proteins ELISA kit (E11R0363)

E11R0363 Bovine Sterol Regulatory Element Binding Proteins ELISA kit

The Bovine Sterol Regulatory Element Binding Proteins ELISA kitt can be used to identify samples from the bovine species. Sterol Regulatory Element Binding Proteins can also be called SREBP.

Products

Specifications of Bovine Sterol Regulatory Element Binding Proteins ELISA kit

Product Information
Cat. No.	E11R0363
Product Name	Bovine Sterol Regulatory Element Binding Proteins ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	1.0-25 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

SREBP ELISA kit uses an anti-SREBP antibody and an SREBP-HRP conjugate in a competitive enzyme immunoassay method. SREBP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-SREBP antibody binding site between SREBP from samples and SREBP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of SREBP. Since the number of sites is limited, as more sites are occupied by SREBP from the sample, fewer sites are left to bind SREBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SREBP concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Sterol Regulatory Element Binding Proteins ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between SREBP and analogues was observed.