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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Triiodothyronine ELISA kit (E11T0039)

E11T0039 Bovine Triiodothyronine ELISA kit

The Bovine Triiodothyronine ELISA kit can be used to identify samples from the bovine species. Triiodothyronine  can also be called 3,3',5-Triiodo-L-Thyronine, Liothyronine, Cytomel, Tertroxin, T3.

Products

Specifications of Bovine Triiodothyronine ELISA kit

Product Information

Cat. No.

E11T0039

Product Name

Bovine Triiodothyronine ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

100-2500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

100 ng/mL

1 vial

STANDARD C (0.5mL)

250 ng/mL

1 vial

STANDARD D (0.5mL)

500 ng/mL

1 vial

STANDARD E (0.5mL)

1000 ng/mL

1 vial

STANDARD F (0.5mL)

2500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

T3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for T3. Standards or samples are then added to the microtiter plate wells and T3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of T3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for T3 are added to each well to “sandwich” the T3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain T3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The T3 concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Triiodothyronine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between T3 and analogues was observed.


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Summary of the Assay Procedure for Bovine Triiodothyronine ELISA kit

Summary of the Assay Procedure for Bovine Triiodothyronine ELISA kit

Citations of Bovine Triiodothyronine ELISA kit

E11T0039 has been referenced in the below publications:

Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.

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