E11T0039 Bovine Triiodothyronine ELISA kit
The Bovine Triiodothyronine ELISA kit can be used to identify samples from the bovine species. Triiodothyronine can also be called 3,3',5-Triiodo-L-Thyronine, Liothyronine, Cytomel, Tertroxin, T3.
Specifications of Bovine Triiodothyronine ELISA kit
Product Information | |
Cat. No. | E11T0039 |
Product Name | Bovine Triiodothyronine ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 100 ng/mL | 1 vial |
STANDARD C (0.5mL) | 250 ng/mL | 1 vial |
STANDARD D (0.5mL) | 500 ng/mL | 1 vial |
STANDARD E (0.5mL) | 1000 ng/mL | 1 vial |
STANDARD F (0.5mL) | 2500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
T3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for T3. Standards or samples are then added to the microtiter plate wells and T3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of T3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for T3 are added to each well to “sandwich” the T3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain T3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The T3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Triiodothyronine ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between T3 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine Triiodothyronine ELISA kit
Citations of Bovine Triiodothyronine ELISA kit
E11T0039 has been referenced in the below publications：
Effect of Different Feeding Patterns on Serum Biochemical Indices and Hormone Content in Dairy Cows.
