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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Bovine Very Low Density Lipoproteins ELISA kit (E11V0006)

E11V0006 Bovine Very Low Density Lipoproteins ELISA kit

The Bovine Very Low Density Lipoproteins ELISA kit can be used to identify samples from the bovine species. Very Low Density Lipoproteins can also be called Pre-β-Lipoprotein, Pre-Beta Lipoprotein, VLDL.

Products

Specifications of Bovine Very Low Density Lipoproteins ELISA kit

Product Information

Cat. No.

E11V0006

Product Name

Bovine Very Low Density Lipoproteins ELISA kit

Species

Bovine

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ug/mL

Sensitivity

0.1 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

VLDL ELISA kit uses an anti-VLDL antibody and an VLDL-HRP conjugate in a competitive enzyme immunoassay method. VLDL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VLDL antibody binding site between VLDL from samples and VLDL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VLDL. Since the number of sites is limited, as more sites are occupied by VLDL from the sample, fewer sites are left to bind VLDL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VLDL concentration in each sample is interpolated from this standard curve.


Quality Control on Bovine Very Low Density Lipoproteins ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between VLDL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Very Low Density Lipoproteins ELISA kit

Summary of the Assay Procedure for Bovine Very Low Density Lipoproteins ELISA kit

Citations of Bovine Very Low Density Lipoproteins ELISA kit

E11V0006 has been referenced in the below publications:

The effects of adenovirus-mediated SREBP-1c gene overexpression on fat deposit in calf hepatocytes cultured in vitro.

SREBP-1c Gene Silencing can Decrease Lipid Deposits in Bovine Hepatocytes Cultured in Vitro.

Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro.

Knockdown of phosphatase and tensin homolog (PTEN) inhibits fatty acid oxidation and reduces very low density lipoprotein assembly and secretion in calf hepatocytes.

Effects of insulin-like growth factor-1 on the assembly and secretion of very low-density lipoproteins in cow hepatocytes in vitro.

SREBP-1c overexpression induces triglycerides accumulation through increasing lipid synthesis and decreasing lipid oxidation and VLDL assembly in bovine hepatocytes.

Acetoacetic acid induces oxidative stress to inhibit the assembly of very low density lipoprotein in bovine hepatocytes.

Glucagon attenuates lipid accumulation in cow hepatocytes through AMPK signaling pathway activation.

FGF21 Reduces Lipid Accumulation in Bovine Hepatocytes by Enhancing Lipid Oxidation and Reducing Lipogenesis via AMPK Signaling.

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