E11V0032 Bovine Vitamin E ELISA kit
The Bovine Vitamin E ELISA kit can be used to identify samples from the bovine species. Vitamin E can also be called Vitamin E, VE.
Specifications of Bovine Vitamin E ELISA kit
Product Information | |
Cat. No. | E11V0032 |
Product Name | Bovine Vitamin E ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 mg/L |
Sensitivity | 1.0 mg/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 mg/L | 1 vial |
STANDARD B (0.5mL) | 5.0 mg/L | 1 vial |
STANDARD C (0.5mL) | 10 mg/L | 1 vial |
STANDARD D (0.5mL) | 25 mg/L | 1 vial |
STANDARD E (0.5mL) | 50 mg/L | 1 vial |
STANDARD F (0.5mL) | 100 mg/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
VE ELISA kit uses an anti-VE antibody and an VE-HRP conjugate in a competitive enzyme immunoassay method. VE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-VE antibody binding site between VE from samples and VE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of VE. Since the number of sites is limited, as more sites are occupied by VE from the sample, fewer sites are left to bind VE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VE concentration in each sample is interpolated from this standard curve. |
Quality Control on Bovine Vitamin E ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between VE and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Bovine Vitamin E ELISA kit
Citations of Bovine Vitamin E ELISA kit
E11V0032 has been referenced in the below publications：
The effect of dietary selenium addition on the concentrations of heavy metals in the tissues of fallow deer (Dama dama L.) in Croatia.
