E01N0028 Human Na+/Ca2+exchanger ELISA kit
The Human Na+/Ca2+exchanger ELISA kit can be used to identify samples from the human species. Na+/Ca2+exchanger can also be called sodium-calcium exchanger.
Specifications of Human Na+/Ca2+exchanger ELISA kit
Product Information | |
Cat. No. | E01N0028 |
Product Name | Human Na+/Ca2+exchanger ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| NCX ELISA kit uses an anti-NCX antibody and an NCX-HRP conjugate in a competitive enzyme immunoassay method. NCX-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NCX antibody binding site between NCX from samples and NCX-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NCX. Since the number of sites is limited, as more sites are occupied by NCX from the sample, fewer sites are left to bind NCX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NCX concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Na+/Ca2+exchanger ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between NCX and analogues was observed. | |
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Summary of the Assay Procedure for Human Na+/Ca2+exchanger ELISA kit
Citations of Human Na+/Ca2+exchanger ELISA kit
E01N0028 has been referenced in the below publications：
