E12C0903 Chicken Ca2+/calmodulin dependent protein kinase ELISA kit
Chicken Ca2+/calmodulin dependent protein kinase ELISA kit is suitable for the detection of samples from chicken species. Ca2+/calmodulin dependent protein kinase can also be called CAMK, CCaMK, Ca2+/calmodulin-dependent protein kinase,CaMK, reproendothelin-1, PPET1, CaM kinase.
Product Information | |
Cat. No. | E12C0903 |
Product Name | Chicken Ca2+/calmodulin dependent protein kinase ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CaM kinase ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CaM kinase antibody and an CaM kinase-HRP conjugate. The assay sample and buffer are incubated together with CaM kinase-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CaM kinase concentration since CaM kinase from samples and CaM kinase-HRP conjugate compete for the anti-CaM kinase antibody binding site. Since the number of sites is limited, as more sites are occupied by CaM kinase from the sample, fewer sites are left to bind CaM kinase-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CaM kinase concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CaM kinase and analogues was observed. | |
E12C0903 has been referenced in the below publications:
Study on Acertain purity of traditional Chinese medicine compound polysaccharides with different MHC B-LβII genotype of chicken immunomodulatory effects receptor TLR2, TLR4 and Immune Signaling Molecule.
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