E02C0682 Rat Calcium/calmodulin dependent protein kinase II ELISA kit
The Rat Calcium/calmodulin dependent protein kinase II ELISA kit can be used to identify samples from the rat species. Calcium/calmodulin dependent protein kinase II can also be called CAMK2; CaM kinase II; CaMKII.
Product Information | |
Cat. No. | E02C0682 |
Product Name | Rat Calcium/calmodulin dependent protein kinase II ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CAMK2 ELISA kit uses an anti-CAMK2 antibody and an CAMK2-HRP conjugate in a competitive enzyme immunoassay method. CAMK2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CAMK2 antibody binding site between CAMK2 from samples and CAMK2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CAMK2. Since the number of sites is limited, as more sites are occupied by CAMK2 from the sample, fewer sites are left to bind CAMK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CAMK2 and analogues was observed. | |
E02C0682 has been referenced in the below publications：
Experimental study of calmodulin-dependent protein kinase II and extracellular signal regulated kinase on the impact of erectile dysfunction in rats with hyperlipidemia.
Related Bluegene Biotech Products
BlueGene Biotech News
