Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit (E08P0188)

BlueGene Biotech Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit (E08P0188)

E08P0188 Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit

The Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit can be used to identify samples from the Canine species. Adenosine Monophosphate Activated Protein Knase α2 can also be called 5'-AMP-activated protein kinase catalytic subunit alpha-2, AAPK2_HUMAN, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK alpha 2 chain, AMPK subunit alpha-2, AMPK2, AMPK 2, AMPKa2, AMPK a2, AMPK-a2, AMPKalpha2, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA, PRKAA2, AMPKα2.

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Specifications of Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit

Product Information
Cat. No.	E08P0188
Product Name	Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit
Species	Canine
Product Size	48 Tests / 96 Tests
Concentration	0.5-10ng/mL
Sensitivity	0.1ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	0.5 ng/mL	1 vial
STANDARD C (0.5mL)	1.0 ng/mL	1 vial
STANDARD D (0.5mL)	2.5 ng/mL	1 vial
STANDARD E (0.5mL)	5.0 ng/mL	1 vial
STANDARD F (0.5mL)	10 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

AMPKα2 ELISA kit uses an anti-AMPKα2 antibody and an AMPKα2-HRP conjugate in a competitive enzyme immunoassay method. AMPKα2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-AMPKα2 antibody binding site between AMPKα2 from samples and AMPKα2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of AMPKα2. Since the number of sites is limited, as more sites are occupied by AMPKα2 from the sample, fewer sites are left to bind AMPKα2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPKα2 concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between AMPKα2 and analogues was observed.

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Summary of the Assay Procedure for Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit

Citations of Canine Adenosine Monophosphate Activated Protein Knase α2 ELISA kit

E08P0188 has been referenced in the below publications：

Epigallocatechin-3-gallate activates the AMP-activated protein kinase signaling pathway to reduce lipid accumulation in canine hepatocytes.