E08H1412 Canine Hyaluronan synthase 2 ELISA Kit
The Canine Hyaluronan synthase 2 ELISA Kit can be used to identify samples from the Canine species. Hyaluronan synthase 2 can also be called HA synthase 2, HAS2_HUMAN, Hyaluronan synthase 2, Hyaluronate synthase 2, Hyaluronic acid synthase 2, HAS2, HAS 2, HAS-2.
Product Information | |
Cat. No. | E08H1412 |
Product Name | Canine Hyaluronan synthase 2 ELISA Kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 25-500ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 25 ng/mL | 1 vial |
STANDARD C (0.5mL) | 50 ng/mL | 1 vial |
STANDARD D (0.5mL) | 100 ng/mL | 1 vial |
STANDARD E (0.5mL) | 250 ng/mL | 1 vial |
STANDARD F (0.5mL) | 500 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HAS2 ELISA kit uses an anti-HAS2 antibody and an HAS2-HRP conjugate in a competitive enzyme immunoassay method. HAS2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HAS2 antibody binding site between HAS2 from samples and HAS2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HAS2. Since the number of sites is limited, as more sites are occupied by HAS2 from the sample, fewer sites are left to bind HAS2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAS2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HAS2 and analogues was observed. | |
E08H1412 has been referenced in the below publications：
Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.
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