E08M0016 Canine Matrix Metalloproteinase 3 ELISA kit
The Canine Matrix Metalloproteinase 3 ELISA kit is suitable for the detection of samples from Canine species. Matrix Metalloproteinase 3 can also be called SL1, STMY, STMY1, STR1, Progelatinase, Stromelysin 1, Transin-1, MMP3, MMP 3, MMP-3.
Product Information | |
Cat. No. | E08M0016 |
Product Name | Canine Matrix Metalloproteinase 3 ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MMP3 ELISA kit uses an anti-MMP3 antibody and an MMP3-HRP conjugate in a competitive enzyme immunoassay method. MMP3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MMP3 antibody binding site between MMP3 from samples and MMP3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MMP3. Since the number of sites is limited, as more sites are occupied by MMP3 from the sample, fewer sites are left to bind MMP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP3 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 90-104% | |
Linearity | Diluent Ratio | Range % |
1:2 | 89-105 | |
1:4 | 87-106 | |
1:8 | 84-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MMP3 and analogues was observed. | |
E08M0016 has been referenced in the below publications:
Articular Cartilage Gene Expression after Coxofemoral Joint Luxation in the Dog.
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