E08M0005 Canine Melatonin ELISA kit
The Canine Melatonin ELISA kit can be used to identify samples from the Canine species. Melatonin can also be called MLT.
Specifications of Canine Melatonin ELISA kit
Product Information | |
Cat. No. | E11I0345 |
Product Name | Canine Melatonin ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MLT ELISA kit uses an anti-MLT antibody and an MLT-HRP conjugate in a competitive enzyme immunoassay method. MLT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-MLT antibody binding site between MLT from samples and MLT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of MLT. Since the number of sites is limited, as more sites are occupied by MLT from the sample, fewer sites are left to bind MLT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLT concentration in each sample is interpolated from this standard curve. |
Quality Control on Canine Melatonin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MLT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Canine Melatonin ELISA kit
Citations of Canine Melatonin ELISA kit
E08M0005 has been referenced in the below publications：
Serum melatonin in dogs with congenital portosystemic shunting, with and without hepatic encephalopathy.
