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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Canine Neuron Specific Enolase ELISA kit (E08N0025)

E08N0025 Canine Neuron Specific Enolase ELISA kit

The Canine Neuron Specific Enolase ELISA kit can be used to identify samples from the Canine species. Neuron Specific Enolase can also be called Gamma-enolase, Gamma enolase, Neural enolase, Neuron specific enolase, neurone-specific enolase, 2 phospho D glycerate hydrolyase, Eno 2, Eno2, ENO2, ENOG, Enolase 2, Enolase 2 gamma neuronal, Enolase2, Neuron specific enolase, Neuron specific gamma enolase, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, enolase 2, gamma, neuronal, NSE.

Products

Specifications of Canine Neuron Specific Enolase ELISA kit

Product Information

Cat. No.

E08N0025

Product Name

Canine Neuron Specific Enolase ELISA kit

Species

Canine

Product Size

48 Tests / 96 Tests

Concentration

250-5000pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NSE ELISA kit uses an anti-NSE antibody and an NSE-HRP conjugate in a competitive enzyme immunoassay method. NSE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NSE antibody binding site between NSE from samples and NSE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NSE. Since the number of sites is limited, as more sites are occupied by NSE from the sample, fewer sites are left to bind NSE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NSE concentration in each sample is interpolated from this standard curve.


Quality Control on Canine Neuron Specific Enolase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NSE and analogues was observed.


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Summary of the Assay Procedure for Canine Neuron Specific Enolase ELISA kit

Summary of the Assay Procedure for Canine Neuron Specific Enolase ELISA kit

Citations of Canine Neuron Specific Enolase ELISA kit

E08N0025 has been referenced in the below publications:

Changes of blood pressure and S-100B, neuron specific enolase protein in hypertensive dogs after renal sympathetic denervation.

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