Canine Neuron Specific Enolase ELISA kit (E08N0025) - Shanghai BlueGene Biotech CO., LTD.

BlueGene Biotech Canine Neuron Specific Enolase ELISA kit (E08N0025)

Neurobiology

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E08N0025 Canine Neuron Specific Enolase ELISA kit

The Canine Neuron Specific Enolase ELISA kit can be used to identify samples from the Canine species. Neuron Specific Enolase can also be called Gamma-enolase, Gamma enolase, Neural enolase, Neuron specific enolase, neurone-specific enolase, 2 phospho D glycerate hydrolyase, Eno 2, Eno2, ENO2, ENOG, Enolase 2, Enolase 2 gamma neuronal, Enolase2, Neuron specific enolase, Neuron specific gamma enolase, Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, enolase 2, gamma, neuronal, NSE.

Products

Specifications of Canine Neuron Specific Enolase ELISA kit

Product Information
Cat. No.	E08N0025
Product Name	Canine Neuron Specific Enolase ELISA kit
Species	Canine
Product Size	48 Tests / 96 Tests
Concentration	250-5000pg/ml
Sensitivity	1.0 pg/ml
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 pg/mL	1 vial
STANDARD B (0.5mL)	250 pg/mL	1 vial
STANDARD C (0.5mL)	500 pg/mL	1 vial
STANDARD D (0.5mL)	1000 pg/mL	1 vial
STANDARD E (0.5mL)	2500 pg/mL	1 vial
STANDARD F (0.5mL)	5000 pg/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

NSE ELISA kit uses an anti-NSE antibody and an NSE-HRP conjugate in a competitive enzyme immunoassay method. NSE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NSE antibody binding site between NSE from samples and NSE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NSE. Since the number of sites is limited, as more sites are occupied by NSE from the sample, fewer sites are left to bind NSE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NSE concentration in each sample is interpolated from this standard curve.

Quality Control on Canine Neuron Specific Enolase ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between NSE and analogues was observed.