E08P0182 Canine Peptidoglycan ELISA kit
The Canine Peptidoglycan ELISA kit can be used to identify samples from the Canine species. Peptidoglycan can also be called Murein, PTG.
Specifications of Canine Peptidoglycan ELISA kit
Product Information | |
Cat. No. | E08P0182 |
Product Name | Canine Peptidoglycan ELISA kit |
Species | Canine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
PTG ELISA kit uses an anti-PTG antibody and an PTG-HRP conjugate in a competitive enzyme immunoassay method. PTG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PTG antibody binding site between PTG from samples and PTG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PTG. Since the number of sites is limited, as more sites are occupied by PTG from the sample, fewer sites are left to bind PTG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTG concentration in each sample is interpolated from this standard curve. |
Quality Control on Canine Peptidoglycan ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between PTG and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Canine Peptidoglycan ELISA kit
Citations of Canine Peptidoglycan ELISA kit
E08P0182 has been referenced in the below publications：
Toll-like receptor mediated activation is possibly involved in immunoregulating properties of cow’s milk hydrolysates.
