CH-K0018-3-Ab Anti-CHO Host Cell Protein G3 Antibody has been validated by ELISA and 2D-WB methods, demonstrating high affinity for HCP, broad HCP coverage, and CHO cell specificity. It can be used for bridging experiments or quality control testing during production.
Specifications Of CH-K0018-3-Ab Anti-CHO Host Cell Protein G3 Antibody
| Product Information | |
Catalog Number | CH-K0018-3-Ab |
Package Size | 100μg |
| Type | Sheep polyclonal antibody IgG |
| Reactive Species | CHO |
| Purity | ≥95% |
| Physical Property | Liquid |
| Components | 0.01M PBS+50% glycerol, 0.3‰ Prolin300, sterile solution |
| Storage | -20℃, Aliquoting is recommended for storage |
| Applications | ELISA, WB, Low-adsorption, High-throughput Protein Chip System, LC-MS |
| Coverage | ≥70% (validated by 2D-WB method) |
| Shelf Life | 3 Years |
Product Introduction |
CHO cell lines are widely used to express therapeutic proteins. During the expression of the target protein, apoptosis of the host cells often occurs. Studies have shown that after cell lysis, hundreds of host cell proteins are released into the culture medium, many of which have strong immunogenicity. This can lead to adverse toxicity or immune reactions, jeopardizing the safety and quality of the product and potentially causing biological contamination. One of the objectives of downstream processes in biopharmaceutical production is to remove these potential hazards. Therefore, it is essential to minimize the residual host cell protein to(HCP) levels. When developing downstream purification processes, a scientifically sound and rational method for qualitatively assessing HCP in the final or semi-product must be in place. This antibody has been validated by ELISA and 2D-WB methods, demonstrating high affinity for HCP, broad HCP coverage, and CHO cell specificity. It can be used for bridging experiments or quality control testing during production. |
