E12M0445 Chicken 72 kDa type IV collagenase ELISA kit
Chicken 72 kDa type IV collagenase ELISA kit is suitable for the detection of samples from Chicken species. 72 kDa type IV collagenase can also be called MMP2, MMP 2, 72 kDa gelatinase, 72kD type IV collagenase, CLG 4, CLG 4A, CLG4, CLG4A, Collagenase Type 4 alpha, Collagenase type IV A, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil.
Product Information | |
Cat. No. | E12M0445 |
Product Name | Chicken 72 kDa type IV collagenase ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MMP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MMP2 antibody and an MMP2-HRP conjugate. The assay sample and buffer are incubated together with MMP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP2 concentration since MMP2 from samples and MMP2-HRP conjugate compete for the anti-MMP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP2 from the sample, fewer sites are left to bind MMP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MMP2 and analogues was observed. | |
E12M0445 has been referenced in the below publications:
Roles of miRNA and MDV oncogene Meq in the transformation of chicken Marek's disease lymphoma.
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