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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Alpha Smooth Muscle Actin ELISA kit (E12S0004)

E12S0004 Chicken Alpha Smooth Muscle Actin ELISA kit

Chicken Alpha Smooth Muscle Actin ELISA kit is suitable for the detection of samples from chicken species. Alpha Smooth Muscle Actin can also be called AAT6, ACTSA, MYMY5, Alpha SMA, AlphaSMA, αSMA, α SMA.

Products

Specifications of Chicken Alpha Smooth Muscle Actin ELISA kit

Product Information

Cat. No.

E12S0004

Product Name

Chicken Alpha Smooth Muscle Actin ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ug/mL

Sensitivity

0.1 ug/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ug/mL

1 vial

STANDARD B (0.5mL)

0.5 ug/mL

1 vial

STANDARD C (0.5mL)

1.0 ug/mL

1 vial

STANDARD D (0.5mL)

2.5 ug/mL

1 vial

STANDARD E (0.5mL)

5.0 ug/mL

1 vial

STANDARD F (0.5mL)

10 ug/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Alpha  SMA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Alpha  SMA antibody and an Alpha  SMA-HRP conjugate. The assay sample and buffer are incubated together with Alpha  SMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Alpha  SMA concentration since Alpha  SMA from samples and Alpha  SMA-HRP conjugate compete for the anti-Alpha  SMA antibody binding site. Since the number of sites is limited, as more sites are occupied by Alpha  SMA from the sample, fewer sites are left to bind Alpha  SMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Alpha  SMA concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Alpha Smooth Muscle Actin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Alpha  SMA  and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Alpha Smooth Muscle Actin ELISA kit

Summary of the Assay Procedure for Chicken Alpha Smooth Muscle Actin ELISA kit

Citations of Chicken Alpha Smooth Muscle Actin ELISA kit

E12S0004 has been referenced in the below publications:

The Effect of the Conlpound Recipe of the Traditional Chinese Medicine on Collagen in Liver of Broilers with Ascites Syndrome.

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