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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Anti Endothelin 1 ELISA kit (E12E0177)

E12E0177 Chicken Anti Endothelin 1 ELISA kit

Chicken Anti Endothelin 1 ELISA kit is suitable for the detection of samples from Chicken species. Endothelin 1 can also be called EDN1, ARCND3, ET1, HDLCQ7, QME, endothelin 1, PPET1,ET 1.

Products

Specifications of Chicken Anti Endothelin 1 ELISA kit

Product Information

Cat. No.

E12E0177

Product Name

Chicken Anti Endothelin 1 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ET 1-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing a ET 1 antigen and an ET 1-Ab-HRP conjugate. The assay sample and buffer are incubated together with ET 1-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ET 1-Ab concentration since ET 1-Ab from samples and ET 1-Ab-HRP conjugate compete for the ET 1 antigen binding site. Since the number of sites is limited, as more sites are occupied by ET 1-Ab from the sample, fewer sites are left to bind ET 1-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ET 1-Ab concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Anti Endothelin 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between anti ET 1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Anti Endothelin 1 ELISA kit

Summary of the Assay Procedure for Chicken Anti Endothelin 1 ELISA kit

Citations of Chicken Anti Endothelin 1 ELISA kit

E12E0177 has been referenced in the below publications:

Multifactorial methods construct ascites syndrome of broilers and protective effect of vascular endothelial function from qi ling gui fu fang for the disease.


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