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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Collagen Type Ⅲ ELISA kit (E12C0017)

E12C0017 Chicken Collagen Type Ⅲ ELISA kit

Chicken Collagen Type Ⅲ ELISA kit is suitable for the detection of samples from Chicken species. Collagen Type Ⅲ can also be called COL3A1, Collagen alpha 1(III) chain, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, COLIII.

Products

Specifications of Chicken Collagen Type Ⅲ ELISA kit

Product Information

Cat. No.

E12C0017

Product Name

Chicken Collagen Type Ⅲ ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

COLIII ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COLIII antibody and an COLIII-HRP conjugate. The assay sample and buffer are incubated together with COLIII-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COLIII concentration since COLIII from samples and COLIII-HRP conjugate compete for the anti-COLIII antibody binding site. Since the number of sites is limited, as more sites are occupied by COLIII from the sample, fewer sites are left to bind COLIII-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COLIII concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Collagen Type Ⅲ ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between COLIII and analogues was observed.


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Summary of the Assay Procedure for Chicken Collagen Type Ⅲ ELISA kit

Summary of the Assay Procedure for Chicken Collagen Type Ⅲ ELISA kit

Citations of Chicken Collagen Type Ⅲ ELISA kit

E12C0017 has been referenced in the below publications:

The Effect of the Conlpound Recipe of the Traditional Chinese Medicine on Collagen in Liver of Broilers with Ascites Syndrome.


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