Name: Chicken Collagen Type I ELISA kit (E12C0014)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Chicken Collagen Type I ELISA kit (E12C0014)

E12C0014 Chicken Collagen Type I ELISA kit

Chicken Collagen Type I ELISA kit is suitable for the detection of samples from Chicken species. Collagen Type I can also be called Collagen type I, Alpha 1 type I collagen, COL1A1, Collagen I alpha 1 polypeptide, Collagen Of Skin Tendon And Bone, Collagen Type 1, Collagen type I alpha 1, Pro alpha 1(I) collagen, Type I procollagen, Collagen alpha-1(II) chain, Collagen alpha-1(II) chain, Chondrocalcin, collagen alpha-1(I) chain preproprotein, COL1, COL 1.

Products

Specifications of Chicken Collagen Type I ELISA kit

Product Information
Cat. No.	E12C0014
Product Name	Chicken Collagen Type I ELISA kit
Species	Chicken
Product Size	48 Tests / 96 Tests
Concentration	50-1000 ng/mL
Sensitivity	1.0 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	50 ng/mL	1 vial
STANDARD C (0.5mL)	100 ng/mL	1 vial
STANDARD D (0.5mL)	250 ng/mL	1 vial
STANDARD E (0.5mL)	500 ng/mL	1 vial
STANDARD F (0.5mL)	1000 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

COL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COL1 antibody and an COL1-HRP conjugate. The assay sample and buffer are incubated together with COL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL1 concentration since COL1 from samples and COL1-HRP conjugate compete for the anti-COL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL1 from the sample, fewer sites are left to bind COL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL1 concentration in each sample is interpolated from this standard curve.

Quality Control on Chicken Collagen Type I ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	91-105%
Linearity	Diluent Ratio	Range %
	1:2	88-106
	1:4	87-108
	1:8	85-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between COL1 and analogues was observed.