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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken cyclic Adenosine Monophosphate ELISA kit (E12C0027)

E12C0027 Chicken cyclic Adenosine Monophosphate ELISA kit

Chicken cyclic Adenosine Monophosphate ELISA kit is suitable for the detection of samples from Chicken species. Cyclic Adenosine Monophosphate can also be called cAMP, cyclic AMP, 3',5'-cyclic adenosine monophosphate.

Products

Specifications of Chicken cyclic Adenosine Monophosphate ELISA kit

Product Information

Cat. No.

E12C0027

Product Name

Chicken cyclic Adenosine Monophosphate ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

10-250pmol/mL

Sensitivity

1.0 pmol/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 pmol/mL

1 vial

STANDARD B (0.5mL)

5.0 pmol/mL

1 vial

STANDARD C (0.5mL)

10 pmol/mL

1 vial

STANDARD D (0.5mL)

50 pmol/mL

1 vial

STANDARD E (0.5mL)

100 pmol/mL

1 vial

STANDARD F (0.5mL)

250 pmol/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

cAMP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-cAMP antibody and an cAMP-HRP conjugate. The assay sample and buffer are incubated together with cAMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cAMP concentration since cAMP from samples and cAMP-HRP conjugate compete for the anti-cAMP antibody binding site. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken cyclic Adenosine Monophosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between cAMP and analogues was observed.


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Summary of the Assay Procedure for Chicken cyclic Adenosine Monophosphate ELISA kit

Summary of the Assay Procedure for Chicken cyclic Adenosine Monophosphate ELISA kit

Citations of Chicken cyclic Adenosine Monophosphate ELISA kit

E12C0027 has been referenced in the below publications:

Study on Acertain purity of traditional Chinese medicine compound polysaccharides with different MHC B-LβII genotype of chicken immunomodulatory effects receptor TLR2, TLR4 and Immune Signaling Molecule.

Effects of Osthole on Progesterone Secretion in Chicken Preovulatory Follicles Granulosa Cells.

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