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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit (E12E0406)

E12E0406 Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit

Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit is suitable for the detection of samples from chicken species. Epidermal growth factor Like Domain Protein, Multiple 7 can also be called EGF like domain 7, EGF like domain containing protein 7, EGF like domain multiple 7, EGFL 7, Epidermal growth factor like domain protein 7, EGFL7.

Products

Specifications of Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit

Product Information

Cat. No.

E12E0406

Product Name

Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

EGFL7 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EGFL7 antibody and an EGFL7-HRP conjugate. The assay sample and buffer are incubated together with EGFL7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EGFL7 concentration since EGFL7 from samples and EGFL7-HRP conjugate compete for the anti-EGFL7 antibody binding site. Since the number of sites is limited, as more sites are occupied by EGFL7 from the sample, fewer sites are left to bind EGFL7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EGFL7 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between EGFL7  and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit

Summary of the Assay Procedure for Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit

Citations of Chicken Epidermal growth factor Like Domain Protein, Multiple 7 ELISA kit

E12E0406 has been referenced in the below publications:

The Effect of the Compound Traditional Chinese Medicine on Endothelium Derived Multiplication Factor in Lung of Broilers with Ascites Syndrome.

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