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  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Glutamine synthetase ELISA kit (E12G0012)

E12G0012 Chicken Glutamine synthetase ELISA kit

Chicken Glutamine synthetase ELISA kit is suitable for the detection of samples from chicken species. Glutamine synthetase can also be called GLNS, GLUL, Glutamate-Ammonia Ligase, Glutamate decarboxylase, GS.

Products

Specifications of Chicken Glutamine synthetase ELISA kit

Product Information

Cat. No.

E12G0012

Product Name

Chicken Glutamine synthetase ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

GS ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GS antibody and an GS-HRP conjugate. The assay sample and buffer are incubated together with GS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GS concentration since GS from samples and GS-HRP conjugate compete for the anti-GS antibody binding site. Since the number of sites is limited, as more sites are occupied by GS from the sample, fewer sites are left to bind GS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GS concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Glutamine synthetase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between GS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Glutamine synthetase ELISA kit

Summary of the Assay Procedure for Chicken Glutamine synthetase ELISA kit

Citations of Chicken Glutamine synthetase ELISA kit

E12G0012 has been referenced in the below publications:

Expression profile of adiponectin and adiponectin receptors in high-fat diet feeding chickens.

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