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  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Matrix metalloproteinase 9 ELISA kit (E12M0329)

E12M0329 Chicken Matrix metalloproteinase 9 ELISA kit

Chicken Matrix metalloproteinase 9 ELISA kit is suitable for the detection of samples from Chicken species. Matrix metalloproteinase 9 can also be called Matrix metalloproteinase-9 precursor, 92 kDa type IV. Collagenase, 92 kDa gelatinase, Gelatinase B, GELB, MMP9, MMP 9, MMP-9. 

Products

Specifications of Chicken Matrix metalloproteinase 9 ELISA kit

Product Information

Cat. No.

E12M0329

Product Name

Chicken Matrix metalloproteinase 9 ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MMP 9 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MMP 9 antibody and an MMP 9-HRP conjugate. The assay sample and buffer are incubated together with MMP 9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP 9 concentration since MMP 9 from samples and MMP 9-HRP conjugate compete for the anti-MMP 9 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP 9 from the sample, fewer sites are left to bind MMP 9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP 9 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Matrix metalloproteinase 9 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MMP 9 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Matrix metalloproteinase 9 ELISA kit

Summary of the Assay Procedure for Chicken Matrix metalloproteinase 9 ELISA kit

Citations of Chicken Matrix metalloproteinase 9 ELISA kit

E12M0329 has been referenced in the below publications:

Roles of miRNA and MDV oncogene Meq in the transformation of chicken Marek's disease lymphoma.


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