E12O0001 Chicken Osteocalcin ELISA kit
Chicken Osteocalcin ELISA kit is suitable for the detection of samples from Chicken species. Osteocalcin can also be called Osteocalcin Propeptide, Osteocalcin precursor, BGLAP, Bone gamma carboxyglutamate protein, BGP, Bone gamma carboxyglutamate gla protein osteocalcin, Bone Gla protein, Gamma carboxyglutamic acid containing protein, OCN, OC.
Product Information | |
Cat. No. | E12O0001 |
Product Name | Chicken Osteocalcin ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
OC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OC antibody and an OC-HRP conjugate. The assay sample and buffer are incubated together with OC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OC concentration since OC from samples and OC-HRP conjugate compete for the anti-OC antibody binding site. Since the number of sites is limited, as more sites are occupied by OC from the sample, fewer sites are left to bind OC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OC concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between OC and analogues was observed. | |
E12O0001 has been referenced in the below publications:
Effects of dietary supplementation with the different oil and oil combination on the growth performance and bone metabolism in AA broilers.
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