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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Phospholipase A2, Secreted ELISA kit (E12S0173)

E12S0173 Chicken Phospholipase A2, Secreted ELISA kit

Chicken Phospholipase A2, Secreted ELISA kit is suitable for the detection of samples from Chicken species. Phospholipase A2, Secreted can also be called PLA2G2D, sPLA2S, s-PLA2, sPLA2, SPLASH, Secreted Phospholipase A2, Phosphatidylcholine 2-acylhydrolase 2D, Secretory-type PLA, stroma-associated homolog.

Products

Specifications of Chicken Phospholipase A2, Secreted ELISA kit

Product Information

Cat. No.

E12S0173

Product Name

Chicken Phospholipase A2, Secreted ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

sPLA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sPLA2 antibody and an sPLA2-HRP conjugate. The assay sample and buffer are incubated together with sPLA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sPLA2 concentration since sPLA2 from samples and sPLA2-HRP conjugate compete for the anti-sPLA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by sPLA2 from the sample, fewer sites are left to bind sPLA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sPLA2 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Phospholipase A2, Secreted ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between sPLA2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Phospholipase A2, Secreted ELISA kit

Summary of the Assay Procedure for Chicken Phospholipase A2, Secreted ELISA kit

Citations of Chicken Phospholipase A2, Secreted ELISA kit

E12S0173 has been referenced in the below publications:

EFFECT OF CLINOPTILOLITE ON GUT PROTECTION OF BROILERS AND STUDY OF RELEVANT MECHANISM.


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