E12S0173 Chicken Phospholipase A2, Secreted ELISA kit
Chicken Phospholipase A2, Secreted ELISA kit is suitable for the detection of samples from Chicken species. Phospholipase A2, Secreted can also be called PLA2G2D, sPLA2S, s-PLA2, sPLA2, SPLASH, Secreted Phospholipase A2, Phosphatidylcholine 2-acylhydrolase 2D, Secretory-type PLA, stroma-associated homolog.
Product Information | |
Cat. No. | E12S0173 |
Product Name | Chicken Phospholipase A2, Secreted ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
sPLA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sPLA2 antibody and an sPLA2-HRP conjugate. The assay sample and buffer are incubated together with sPLA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sPLA2 concentration since sPLA2 from samples and sPLA2-HRP conjugate compete for the anti-sPLA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by sPLA2 from the sample, fewer sites are left to bind sPLA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sPLA2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between sPLA2 and analogues was observed. | |
E12S0173 has been referenced in the below publications:
EFFECT OF CLINOPTILOLITE ON GUT PROTECTION OF BROILERS AND STUDY OF RELEVANT MECHANISM.
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