E12T0023 Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit
Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit is suitable for the detection of samples from chicken species. Tumor necrosis factor related apoptosis inducing ligand can also be called TNFSF10, APO2L, Apo-2L, CD253, TL2, TRAIL, TNLG6A, tumor necrosis factor superfamily member 10, TNF superfamily member 10.
Product Information | |
Cat. No. | E12T0023 |
Product Name | Chicken Tumor necrosis factor related apoptosis inducing ligand ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/ml |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TRAIL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TRAIL antibody and an TRAIL-HRP conjugate. The assay sample and buffer are incubated together with TRAIL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRAIL concentration since TRAIL from samples and TRAIL-HRP conjugate compete for the anti-TRAIL antibody binding site. Since the number of sites is limited, as more sites are occupied by TRAIL from the sample, fewer sites are left to bind TRAIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRAIL concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 88-116.69% | |
Linearity | Diluent Ratio | Range % |
1:2 | 95-117 | |
1:4 | 91-107 | |
1:8 | 89 -104 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TRAIL and analogues was observed. | |
E12T0023 has been referenced in the below publications:
Roles of miRNA and MDV oncogene Meq in the transformation of chicken Marek's disease lymphoma.
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