E01A0001 Human Antioncogene P16 Protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit
Human Antioncogene p16 protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit is suitable for the detection of samples from human species. Antioncogene p16 protein/Cyclin Dependent Kinase Inhibitor 2A can also be called AGPT2, ANGPT2, NG2, angiopoietin 2, and ANG-2, p16.
| Product Information | |
| Cat. No. | E01A0001 |
| Product Name | Human Antioncogene p16 protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit |
| Species | Human |
| Product Size | 48 Tests / 96 Tests |
| Concentration | 2.5-50 ng/mL |
| Sensitivity | 0.1 ng/mL |
| Principal | Competitive ELISA |
| Sample Volume | 100 ul |
| Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
| Assay Time | 90 minutes |
| Platform | Microplate Reader |
| Conjugate | HRP |
| Detection Method | Colorimetric |
| Storage | 2-8°C |
| Kit Components | ||
| MATERIALS | SPECIFICATION | QUANTITY |
| MICROTITER PLATE | 96 wells | stripwell |
| ENZYME CONJUGATE | 6.0 mL | 1 vial |
| STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
| STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
| STANDARD C (0.5mL) | 5 ng/mL | 1 vial |
| STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
| STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
| STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
| SUBSTRATE A | 6 mL | 1 vial |
| SUBSTRATE B | 6 mL | 1 vial |
| STOP SOLUTION | 6 mL | 1 vial |
| WASH SOLUTION (100 x) | 10 mL | 1 vial |
| BALANCE SOLUTION | 3 mL | 1 vial |
| Principle of the Assay | |
| p16 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-p16 antibody and an p16-HRP conjugate. The assay sample and buffer are incubated together with p16-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p16 concentration since p16 from samples and p16-HRP conjugate compete for the anti-p16 antibody binding site. Since the number of sites is limited, as more sites are occupied by p16 from the sample, fewer sites are left to bind p16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p16 concentration in each sample is interpolated from this standard curve. |
| Coefficient of Variance | Intra Variation% ＜10% | |
| Inter Variation% ＜12% | ||
| Recovery | 85-113% | |
| Linearity | Diluent Ratio | Range % |
| 1:2 | 96-105 | |
| 1:4 | 92-107 | |
| 1:8 | 87-112 | |
| Specificity/Cross-reactivity | No significant cross-reactivity or interference between p16 and analogues was observed. | |
E01A0001 has been referenced in the below publications:
Serum p16 and p21 protein expression among people living in Bama county of Guangxi.
The Senescence-Associated Secretory Phenotype Promotes Benign Prostatic Hyperplasia.
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