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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Antioncogene P16 Protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit (E01A0001)

E01A0001 Human Antioncogene P16 Protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit

Human Antioncogene p16 protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit is suitable for the detection of samples from human species. Antioncogene p16 protein/Cyclin Dependent Kinase Inhibitor 2A can also be called AGPT2, ANGPT2, NG2, angiopoietin 2, and ANG-2, p16.

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Specifications of Human Antioncogene P16 Protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit

Product Information
Cat. No.E01A0001
Product NameHuman Antioncogene p16 protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit
SpeciesHuman
Product Size48 Tests / 96 Tests
Concentration2.5-50 ng/mL
Sensitivity0.1 ng/mL
PrincipalCompetitive ELISA
Sample Volume100 ul
Sample TypeSerum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time90 minutes
PlatformMicroplate Reader
ConjugateHRP
Detection MethodColorimetric
Storage2-8°C


Kit Components
MATERIALSSPECIFICATIONQUANTITY
MICROTITER PLATE96 wellsstripwell
ENZYME CONJUGATE6.0 mL1 vial
STANDARD A (0.5mL)0 ng/mL1 vial
STANDARD B (0.5mL)2.5 ng/mL1 vial
STANDARD C (0.5mL)5 ng/mL1 vial
STANDARD D (0.5mL)10 ng/mL1 vial
STANDARD E (0.5mL)25 ng/mL1 vial
STANDARD F (0.5mL)50 ng/mL1 vial
SUBSTRATE A6 mL1 vial
SUBSTRATE B6 mL1 vial
STOP SOLUTION6 mL1 vial
WASH SOLUTION (100 x)10 mL1 vial
BALANCE SOLUTION3 mL1 vial



Principle of the Assay
p16 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-p16 antibody and an p16-HRP conjugate. The assay sample and buffer are incubated together with p16-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p16 concentration since p16 from samples and p16-HRP conjugate compete for the anti-p16 antibody binding site. Since the number of sites is limited, as more sites are occupied by p16 from the sample, fewer sites are left to bind p16-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p16 concentration in each sample is interpolated from this standard curve.



Quality Control on Human Antioncogene P16 Protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit

Coefficient of VarianceIntra Variation% <10%
Inter Variation% <12%
Recovery85-113%
LinearityDiluent RatioRange %
1:296-105
1:492-107
1:887-112
Specificity/Cross-reactivityNo significant cross-reactivity or interference between p16 and analogues was observed.


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Summary of the Assay Procedure for Human Antioncogene P16 Protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit

Summary of the Assay Procedure for Human Antioncogene P16 Protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit

Citations of Human Antioncogene P16 Protein/Cyclin Dependent Kinase Inhibitor 2A ELISA kit

E01A0001 has been referenced in the below publications:

Serum p16 and p21 protein expression among people living in Bama county of Guangxi.

The Senescence-Associated Secretory Phenotype Promotes Benign Prostatic Hyperplasia.

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