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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Adrenocorticotropic Hormone ELISA kit (E01A0005)

E01A0005 Human Adrenocorticotropic Hormone ELISA kit

Human Adrenocorticotropic Hormone ELISA kit is suitable for the detection of samples from human species. Adrenocorticotropic Hormone can also be called POMC, Pro-opiomelanocortin, Corticotropin-lipotropin, Adrenocorticotropic hormone, adrenocorticotropin, corticotropin, Corticotropin, Adrenocorticotropin, ACTH.

Products

Specifications of Human Adrenocorticotropic Hormone ELISA kit

Product Information

Cat. No.

E01A0005

Product Name

Human Adrenocorticotropic Hormone ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ACTH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ACTH antibody and an ACTH-HRP conjugate. The assay sample and buffer are incubated together with ACTH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTH concentration since ACTH from samples and ACTH-HRP conjugate compete for the anti-ACTH antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTH from the sample, fewer sites are left to bind ACTH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTH concentration in each sample is interpolated from this standard curve.


Quality Control on Human Adrenocorticotropic Hormone ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

91-104

1:8

92-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ACTH and analogues was observed.


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Summary of the Assay Procedure for Human Adrenocorticotropic Hormone ELISA kit

Summary of the Assay Procedure for Human Adrenocorticotropic Hormone ELISA kit

Citations of Human Adrenocorticotropic Hormone ELISA kit

E01A0005 has been referenced in the below publications:

The Effects of Smad3 on Adrenocorticotropic HormoneeSecreting Pituitary Adenoma Development, Cell Proliferation, Apoptosis, and Hormone Secretion.

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