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  • bovine serum albumin elisa 1

BlueGene Biotech Human Epinephrine/Adrenaline ELISA kit (E01A0007)

E01A0007 Human Epinephrine/Adrenaline ELISA kit

Human Epinephrine/Adrenaline ELISA kit is suitable for the detection of samples from human species. Epinephrine/Adrenaline can also be called ADR, EPI.

Products

Specifications of Human Epinephrine/Adrenaline ELISA kit

Product Information

Cat. No.

E01A0007

Product Name

Human Epinephrine/Adrenaline ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

EPI ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-EPI antibody and an EPI-HRP conjugate. The assay sample and buffer are incubated together with EPI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EPI concentration since EPI from samples and EPI-HRP conjugate compete for the anti-EPI antibody binding site. Since the number of sites is limited, as more sites are occupied by EPI from the sample, fewer sites are left to bind EPI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EPI concentration in each sample is interpolated from this standard curve.


Quality Control on Human Epinephrine/Adrenaline ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

89-108%

Linearity

Diluent Ratio

Range %

1:2

93-102

1:4

90-105

1:8

89-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between EPI and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Epinephrine/Adrenaline ELISA kit

Summary of the Assay Procedure for Human Epinephrine/Adrenaline ELISA kit

Citations of Human Epinephrine/Adrenaline ELISA kit

E01A0007 has been referenced in the below publications:

The influences of stellate ganglion block on the level of stress hormone for patients of cephalic and facial postherpetic neuralgia.

Effects of hemodialysis on the cardiovascular function in patients with intradialytic hypertension and the related mechanism.

Changes of ET-1 and NO in the maintenance hemodialysis complicated with intradialytic hypertension.

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