Name: Human Active Caspase 3 ELISA kit (E01A0015)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Human Active Caspase 3 ELISA kit (E01A0015)

E01A0015 Human Active Caspase 3 ELISA kit

Human Active caspase 3 ELISA kit is suitable for the detection of samples from human species. Active caspase 3 can also be called CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1, Active casp3, casp3, casp-3, casp 3.

Products

Specifications of Human Active Caspase 3 ELISA kit

Product Information
Cat. No.	E01A0015
Product Name	Human Active caspase 3 ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

Active caspase 3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Active caspase 3 antibody and an Active caspase 3-HRP conjugate. The assay sample and buffer are incubated together with Active caspase 3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Active caspase 3 concentration since Active caspase 3 from samples and Active caspase 3-HRP conjugate compete for the anti-Active caspase 3 antibody binding site. Since the number of sites is limited, as more sites are occupied by Active caspase 3 from the sample, fewer sites are left to bind Active caspase 3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Active caspase 3 concentration in each sample is interpolated from this standard curve.

Quality Control on Human Active Caspase 3 ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	92-109%
Linearity	Diluent Ratio	Range %
	1:2	93-103
	1:4	91-109
	1:8	89-110
Specificity/Cross-reactivity	No significant cross-reactivity or interference between active casp3 and analogues was observed.