En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Active Caspase 3 ELISA kit (E01A0015)

E01A0015 Human Active Caspase 3 ELISA kit

Human Active caspase 3 ELISA kit is suitable for the detection of samples from human species. Active caspase 3 can also be called CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1, Active casp3, casp3, casp-3, casp 3.

Products

Specifications of Human Active Caspase 3 ELISA kit

Product Information

Cat. No.

E01A0015

Product Name

Human Active caspase 3 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial



Principle of the Assay

Active caspase 3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Active caspase 3 antibody and an Active caspase 3-HRP conjugate. The assay sample and buffer are incubated together with Active caspase 3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Active caspase 3 concentration since Active caspase 3 from samples and Active caspase 3-HRP conjugate compete for the anti-Active caspase 3 antibody binding site. Since the number of sites is limited, as more sites are occupied by Active caspase 3 from the sample, fewer sites are left to bind Active caspase 3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Active caspase 3 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Active Caspase 3 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-109%

Linearity

Diluent Ratio

Range %

1:2

93-103

1:4

91-109

1:8

89-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between active casp3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Active Caspase 3 ELISA kit

Summary of the Assay Procedure for Human Active Caspase 3 ELISA kit

Citations of Human Active Caspase 3 ELISA kit

E01A0015 has been referenced in the below publications:

Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.