E01A0031 Human Alkaline Phosphatase ELISA kit
Human Alkaline Phosphatase ELISA kit is suitable for the detection of samples from human species. Alkaline Phosphatase can also be called AKP, ALKP, Basic Phosphatase, ALP.
Specifications of Human Alkaline Phosphatase ELISA kit
Product Information | |
Cat. No. | E01A00031 |
Product Name | Human Alkaline Phosphatase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ALP antibody and an ALP-HRP conjugate. The assay sample and buffer are incubated together with ALP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALP concentration since ALP from samples and ALP-HRP conjugate compete for the anti-ALP antibody binding site. Since the number of sites is limited, as more sites are occupied by ALP from the sample, fewer sites are left to bind ALP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALP concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Alkaline Phosphatase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 89-107% | |
Linearity | Diluent Ratio | Range % |
1:2 | 91-106 | |
1:4 | 90-103 | |
1:8 | 89-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ALP and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Alkaline Phosphatase ELISA kit
Citations of Human Alkaline Phosphatase ELISA kit
E01A0031 Human Alkaline Phosphatase ELISA kit
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