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BlueGene Biotech Human Artemin ELISA kit (E01A0032)

E01A0032 Human Artemin ELISA kit

Human Artemin ELISA kit is suitable for the detection of samples from human species. Artemin can also be called ARTN, ENOVIN, EVN, NBN, artemin, ART.

Products

Specifications of Human Artemin ELISA kit

Product Information

Cat. No.

E01A0032

Product Name

Human Artemin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ART ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ART antibody and an ART-HRP conjugate. The assay sample and buffer are incubated together with ART-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ART concentration since ART from samples and ART-HRP conjugate compete for the anti-ART antibody binding site. Since the number of sites is limited, as more sites are occupied by ART from the sample, fewer sites are left to bind ART-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ART concentration in each sample is interpolated from this standard curve.


Quality Control on Human Artemin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

92-105%

Linearity

Diluent Ratio

Range %

1:2

95-104

1:4

93-108

1:8

94-109

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ART and analogues was observed.



BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Artemin ELISA kit

Summary of the Assay Procedure for Human Artemin ELISA kit

Citations of Human Artemin ELISA kit

E01A0015 has been referenced in the below publications:

Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression.

Acute exercise prevents the development of neuropathic pain and the sprouting of non-peptidergic (GDNF- and arteminresponsive) c-fibers after spinal cord injury.

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