E01A0035 Human Asymmetric Dimethylaoyoinine ELISA kit
Human Asymmetric Dimethylaoyoinine ELISA kit is suitable for the detection of samples from human species. Asymmetric Dimethylaoyoinine can also be called N-Dimethylarginine: NG, NG-Dimethylarginine dihydrochloride, Asymmetric Dimethylarginine, ADMA.
Product Information | |
Cat. No. | E01A0035 |
Product Name | Human Asymmetric Dimethylaoyoinine ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 μmoL/L |
Sensitivity | 0.1 μmoL/L |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 μmoL/L | 1 vial |
STANDARD B (0.5mL) | 2.5 μmoL/L | 1 vial |
STANDARD C (0.5mL) | 5.0 μmoL/L | 1 vial |
STANDARD D (0.5mL) | 10 μmoL/L | 1 vial |
STANDARD E (0.5mL) | 25 μmoL/L | 1 vial |
STANDARD F (0.5mL) | 50 μmoL/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ADMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ADMA. Standards or samples are then added to the microtiter plate wells and ADMA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADMA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADMA are added to each well to “sandwich” the ADMA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ADMA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADMA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-107% | |
Linearity | Diluent Ratio | Range % |
1:2 | 95-102 | |
1:4 | 90-107 | |
1:8 | 93-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ADMA and analogues was observed. | |
E01A0035 has been referenced in the below publications:
Dickkopf1 destabilizes atherosclerotic plaques and promotes plaque formation by inducing apoptosis of endothelial cells through activation of ER stress.
Brain Volumetrics, Regional Cortical Thickness and Radiographic Findings in Adults with Cyanotic Congenital Heart Disease.
Effect of super-flux polyethersulfone dialysis membrane on the removal of asymmetrical dimethylarginine: a pilot clinical study.
The Effect and Mechanism of Asymmetric Dimethylarginine Regulating Trophoblastic Autophagy on Fetal Growth Restriction.
The role and mechanism of asymmetric dimethylarginine in fetal growth restriction via interference with endothelial function and angiogenesis.
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