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BlueGene Biotech Human Adenosine Triphosphate ELISA kit(E01A0038)

E01A0038 Human Adenosine Triphosphate ELISA kit

Human Adenosine Triphosphate ELISA kit is suitable for the detection of samples from human species. Adenosine Triphosphate can also be called Adenosine-5'-Triphosphate, ATP.

Products

Specifications of Human Adenosine Triphosphate ELISA kit

Product Information

Cat. No.

E01A0038

Product Name

Human Adenosine Triphosphate ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are incubated together with ATP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP concentration since ATP from samples and ATP-HRP conjugate compete for the anti-ATP antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP concentration in each sample is interpolated from this standard curve.


Quality Control on Human Adenosine Triphosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-106%

Linearity

Diluent Ratio

Range %

1:2

93-101

1:4

90-107

1:8

92-105

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ATP and analogues was observed.



BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Adenosine Triphosphate ELISA kit

Summary of the Assay Procedure for Human Adenosine Triphosphate ELISA kit

Citations of Human Adenosine Triphosphate ELISA kit

E01A0038 has been referenced in the below publications:

The research of the metabolism and in vivo functional changes of erythrocyte stored at -65℃.

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