Name: Human Adenosine Diphosphate ELISA kit (E01A0044)
Brand: Shanghai BlueGene Biotech CO., LTD.
Price: 100 USD

BlueGene Biotech Human Adenosine Diphosphate ELISA kit (E01A0044)

E01A0044 Human Adenosine Diphosphate ELISA kit

Human Adenosine diphosphate ELISA kit is suitable for the detection of samples from human species. Adenosine Diphosphate is also known as ADP, Adenosine 5'-diphosphate, Adenylpyrophosphate, Adenosine pyrophosphate, Adenosine 5'-pyrophosphate.

Products

Specifications of Human Adenosine Diphosphate ELISA kit

Product Information
Cat. No.	E01A0044
Products Name	Human Adenosine diphosphate ELISA kit
Species	Human
Product Size	48 Tests / 96 Tests
Concentration	1.0-25ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	1.0 ng/mL	1 vial
STANDARD C (0.5mL)	2.5 ng/mL	1 vial
STANDARD D (0.5mL)	5.0 ng/mL	1 vial
STANDARD E (0.5mL)	10 ng/mL	1 vial
STANDARD F (0.5mL)	25 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

ADP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADP antibody and an ADP-HRP conjugate. The assay sample and buffer are incubated together with ADP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADP concentration since ADP from samples and ADP-HRP conjugate compete for the anti-ADP antibody binding site. Since the number of sites is limited, as more sites are occupied by ADP from the sample, fewer sites are left to bind ADP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADP concentration in each sample is interpolated from this standard curve.

Quality Control on Human Adenosine Diphosphate ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	93-103%
Linearity	Diluent Ratio	Range %
	1:2	92-104
	1:4	90-108
	1:8	89-113
Specificity/Cross-reactivity	No significant cross-reactivity or interference between ADP and analogues was observed.