E01A0049 Human Amyloid β Protein 40 ELISA kit
Human Adenosine diphosphate ELISA kit is suitable for the detection of samples from human species. Amyloid β Protein 40 can also be called Aβ40, beta-amyloid 40.
Specifications of Human Amyloid β Protein 40 ELISA kit
Product Information | |
Cat. No. | E01A0049 |
Product Name | Human Amyloid β Protein 40 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AΒ40 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AΒ40. Standards or samples are then added to the microtiter plate wells and AΒ40 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AΒ40 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AΒ40 are added to each well to “sandwich” the AΒ40 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AΒ40 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AΒ40 concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Amyloid β Protein 40 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 96-105 | |
1:4 | 903-103 | |
1:8 | 89-107 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AΒ40 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Amyloid β Protein 40 ELISA kit
Citations of Human Amyloid β Protein 40 ELISA kit
E01A0049 has been referenced in the below publications:
Therapeutic effect of probucol on mild cognitive impairment in type 2 diabetic patients.
Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer's Disease-Like Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus.
