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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human 1,25 dihydroxyvitamin D3 ELISA kit (E01D0002)

E01D0002 Human 1,25 dihydroxyvitamin D3 ELISA kit

Human 1,25 dihydroxyvitamin D3 ELISA kit is suitable for detecting samples from the human species. 1,25 dihydroxy vitamin D3 can also be called 1,25(OH)2D3.

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Specifications of Human 1,25 dihydroxyvitamin D3 ELISA kit

Product Information
Cat. No.E01D0002
Product NameHuman 1,25 dihydroxyvitamin D3 ELISA kit
SpeciesHuman
Product Size48 Tests / 96 Tests
Concentration25-500 pg/mL
Sensitivity1.0 pg/mL
PrincipalCompetitive ELISA
Sample Volume100 ul
Sample TypeSerum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time90 minutes
PlatformMicroplate Reader
ConjugateHRP
Detection MethodColorimetric
Storage2-8°C


Kit Components
MATERIALSSPECIFICATIONQUANTITY
MICROTITER PLATE96 wellsstripwell
ENZYME CONJUGATE6.0 mL1 vial
STANDARD A (0.5mL)0 pg/mL1 vial
STANDARD B (0.5mL)25 pg/mL1 vial
STANDARD C (0.5mL)50 pg/mL1 vial
STANDARD D (0.5mL)100 pg/mL1 vial
STANDARD E (0.5mL)250 pg/mL1 vial
STANDARD F (0.5mL)500 pg/mL1 vial
SUBSTRATE A6 mL1 vial
SUBSTRATE B6 mL1 vial
STOP SOLUTION6 mL1 vial
WASH SOLUTION (100 x)10 mL1 vial
BALANCE SOLUTION3 mL1 vial



Principle of the Assay

1,25 (OH)2D3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-1,25 (OH)2D3 antibody and an 1,25 (OH)2D3-HRP conjugate. The assay sample and buffer are incubated together with 1,25 (OH)2D3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 1,25 (OH)2D3 concentration since 1,25 (OH)2D3 from samples and 1,25 (OH)2D3-HRP conjugate compete for the anti-1,25 (OH)2D3 antibody binding site. Since the number of sites is limited, as more sites are occupied by 1,25 (OH)2D3 from the sample, fewer sites are left to bind 1,25 (OH)2D3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 1,25 (OH)2D3 concentration in each sample is interpolated from this standard curve.



Quality Control on Human 1,25 dihydroxyvitamin D3 ELISA kit

Coefficient of VarianceIntra Variation% <10%
Inter Variation% <12%
Recovery88-118%
LinearityDiluent RatioRange %
1:297-103
1:492-104
1:889-99
Specificity/Cross-reactivityNo significant cross-reactivity or interference between 1,25 (OH)2D3 and analogues was observed.


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Summary of the Assay Procedure for Human 1,25 dihydroxyvitamin D3 ELISA kit

Summary of the Assay Procedure for Human 1,25 dihydroxyvitamin D3 ELISA kit

Citations of Human 1,25 dihydroxyvitamin D3 ELISA kit

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