E02A0004 Rat Antioncogene P21 Protein/Cyclin Dependent Kinase Inhibitor 1 ELISA kit
Rat Antioncogene p21 protein/Cyclin dependent kinase inhibitor 1 ELISA kit is suitable for the detection of samples from Rat species. Antioncogene p21 protein/Cyclin dependent kinase inhibitor 1 can also be called CDK-interacting protein 1, Melanoma differentiation-associated protein 6, CAP20, WAF1/CIP1, SDI1, MDA-6, PIC1, CDKN1, p21Cip1/Waf1, p21Waf1/Cip1, p21.
Product Information | |
Cat. No. | E02A0004 |
Product Name | Rat Antioncogene p21 protein/Cyclin dependent kinase inhibitor 1 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
p21 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-p21 antibody and an p21-HRP conjugate. The assay sample and buffer are incubated together with p21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p21 concentration since p21 from samples and p21-HRP conjugate compete for the anti-p21 antibody binding site. Since the number of sites is limited, as more sites are occupied by p21 from the sample, fewer sites are left to bind p21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p21 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 96-108% | |
Linearity | Diluent Ratio | Range % |
1:2 | 91-105 | |
1:4 | 90-108 | |
1:8 | 87-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between p21 and analogues was observed. | |
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