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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Adenosine Monophosphate ELISA kit (E02A0018)

E02A0018 Rat Adenosine Monophosphate ELISA kit

Rat Adenosine Monophosphate ELISA kit is suitable for the detection of samples from Rat species. Adenosine Triphosphate can also be called adenosine-5'-triphosphate, AMP.

Products

Rat Adenosine Monophosphate ELISA kit

Product Information

Cat. No.

E02A0018

Product Name

Rat Adenosine Monophosphate ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

25-500 pmol/mL

Sensitivity

1.0 pmol/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pmol/mL

1 vial

STANDARD B (0.5mL)

25 pmol/mL

1 vial

STANDARD C (0.5mL)

50 pmol/mL

1 vial

STANDARD D (0.5mL)

100 pmol/mL

1 vial

STANDARD E (0.5mL)

250 pmol/mL

1 vial

STANDARD F (0.5mL)

500 pmol/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AMP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMP antibody and an AMP-HRP conjugate. The assay sample and buffer are incubated together with AMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMP concentration since AMP from samples and AMP-HRP conjugate compete for the anti-AMP antibody binding site. Since the number of sites is limited, as more sites are occupied by AMP from the sample, fewer sites are left to bind AMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Adenosine Monophosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

93-106%

Linearity

Diluent Ratio

Range %

1:2

95-103

1:4

93-106

1:8

91-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AMP and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Adenosine Monophosphate ELISA kit

Summary of the Assay Procedure for Rat Adenosine Monophosphate ELISA kit

Citations of Rat Adenosine Monophosphate ELISA kit

E02A0018 has been referenced in the below publications:

Gualou Xiebai Decoction, a Traditional Chinese Medicine, Prevents Cardiac Reperfusion Injury in Hyperlipidemia Rats via Energy Modulation.

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