E02A0028 Rat Albumin ELISA kit
Rat Albumin ELISA kit is suitable for the detection of samples from Rat species. Albumin can also be called ALB, Serum albumin.
Specifications of Rat Albumin ELISA kit
Product Information | |
Cat. No. | E02A0028 |
Product Name | Rat Albumin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ALB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ALB. Standards or samples are then added to the microtiter plate wells and ALB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ALB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ALB are added to each well to “sandwich” the ALB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ALB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALB concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Albumin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-110% | |
Linearity | Diluent Ratio | Range % |
1:2 | 94-102 | |
1:4 | 92-105 | |
1:8 | 89-113 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ALB and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Albumin ELISA kit
Citations of Rat Albumin ELISA kit
E02A0028 has been referenced in the below publications:
Relationship between urinary nephrin and urinary albumin change- es in diabetic rats and effects of Yiqiyangyinhuayutongluo Recipe.
effects of Chinese Material Medica of qi benefiting, yin nourishing, stasis removing, and collaterals dredging and their dissembled recipes on nephrin of diabetes mellitus model rats.
Effects of the yiqi-yangyin-huoxue-tongluo recipe on podocyte of diabetic nephropathy rats.
The therapeutic effect of poIygonatum sibiricum polysaccharid on diabetic nephropathy rats and the possible mechanism.
