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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Asymmetric Dimethylaoyoinine ELISA kit (E02A0035)

E02A0035 Rat Asymmetric Dimethylaoyoinine ELISA kit

Rat Asymmetric Dimethylaoyoinine ELISA kit is suitable for the detection of samples from Rat species. Asymmetric Dimethylaoyoinine can also be called N-Dimethylarginine, NG, NG-Dimethylarginine dihydrochloride, ADMA.

Products

Specifications of Rat Asymmetric Dimethylaoyoinine ELISA kit

Product Information

Cat. No.

E02A0035

Product Name

Rat Asymmetric Dimethylaoyoinine ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ADMA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADMA antibody and an ADMA-HRP conjugate. The assay sample and buffer are incubated together with ADMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADMA concentration since ADMA from samples and ADMA-HRP conjugate compete for the anti-ADMA antibody binding site. Since the number of sites is limited, as more sites are occupied by ADMA from the sample, fewer sites are left to bind ADMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADMA concentration in each sample is interpolated from this standard curve.



Quality Control on Rat Asymmetric Dimethylaoyoinine ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-109%

Linearity

Diluent Ratio

Range %

1:2

91-103

1:4

92-108

1:8

87-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ADMA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Asymmetric Dimethylaoyoinine ELISA kit

Summary of the Assay Procedure for Rat Asymmetric Dimethylaoyoinine ELISA kit

Citations of Rat Asymmetric Dimethylaoyoinine ELISA kit

E02A0031 has been referenced in the below publications:

Combined Intraperitoneal and Intrathecal Etanercept Reduce Increased Brain Tumor Necrosis Factor-Alpha and Asymmetric Dimethylarginine Levels and Rescues Spatial Deficits in Young Rats after Bile Duct Ligation.

Melatonin prevented spatial deficits and increases in brain asymmetric dimethylarginine in young bile duct ligation rats.

Study of the effect of ADMA on hepatic stellate cells proliferation and its possible signal pathway.

Impact of Rosuvastatin on vascular endothelial function in H-hypertensive rats.


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