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BlueGene Biotech Rat Total β Amyloid Protein ELISA kit (E02A0039)

E02A0039 Rat Total β Amyloid Protein ELISA kit

Rat Total β Amyloid Protein ELISA kit is suitable for the detection of samples from Rat species. Total β Amyloid Protein can also be called Aβ, Aβ protein, Amyloid beta-peptide, Beta-amyloid peptide, Beta-amyloid protein, beta Amyloid Protein.

Products

Specifications of Rat Total β Amyloid Protein ELISA kit

Product Information

Cat. No.

E02A0039

Product Name

Rat Total β Amyloid Protein ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AβELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Aβantibody and an A-HRP conjugate. The assay sample and buffer are incubated together with Aβ-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Aβconcentration since Aβfrom samples and Aβ-HRP conjugate compete for the anti-Aβantibody binding site. Since the number of sites is limited, as more sites are occupied by Aβ from the sample, fewer sites are left to bind Aβ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Aβconcentration in each sample is interpolated from this standard curve.


Quality Control on Rat Total β Amyloid Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

93-106%

Linearity

Diluent Ratio

Range %

1:2

94-104

1:4

92-110

1:8

90-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Aβ and analogues was observed.



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Summary of the Assay Procedure for Rat Total β Amyloid Protein ELISA kit

Summary of the Assay Procedure for Rat Total β Amyloid Protein ELISA kit

Citations of Rat Total β Amyloid Protein ELISA kit

E02A0039 has been referenced in the below publications:

Kai-Xin-San, a Chinese Herbal Decoction, Accelerates the Degradation of β-Amyloid by Enhancing the Expression of Neprilysin in Rats.

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