E02A0043 Rat Adenosine Monophosphate Activated Protein ELISA kit
Rat Adenosine Monophosphate Activated Protein ELISA kit is suitable for the detection of samples from Rat species. Adenosine Monophosphate Activated Protein can also be called MPK, HAMPKb, 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit, AMAP.
Product Information | |
Cat. No. | E02A0028 |
Product Name | Rat Adenosine Monophosphate Activated Protein ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000ng/ml |
Sensitivity | 1.0 ng/mL |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 50 ng/mL | 1 vial |
STANDARD C (0.5mL) | 100 ng/mL | 1 vial |
STANDARD D (0.5mL) | 250 ng/mL | 1 vial |
STANDARD E (0.5mL) | 500 ng/mL | 1 vial |
STANDARD F (0.5mL) | 1000 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
AMPK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AMPK. Standards or samples are then added to the microtiter plate wells and AMPK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AMPK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AMPK are added to each well to "sandwich" the AMPK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AMPK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMPK concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 93-112% | |
Linearity | Diluent Ratio | Range % |
1:2 | 91-102 | |
1:4 | 93-105 | |
1:8 | 90-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between AMPK and analogues was observed. | |
E02A0043 has been referenced in the below publications:
The preliminary study on the effects of AMPK activation induced by curcumin and aerobic exercise on middle-aged rats' skeletal muscle cellautophagy.
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