E03A0019 Mouse Androgen ELISA kit
The Mouse Androgen ELISA kit is suitable for detecting samples from mouse species. Androgen can also be called ADG, testosterone hormone.
Specifications of Mouse Androgen ELISA kit
Product Information | |
Cat. No. | E03A0019 |
Product Name | Mouse Androgen ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ADG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ADG antibody and an ADG-HRP conjugate. The assay sample and buffer are incubated together with ADG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADG concentration since ADG from samples and ADG-HRP conjugate compete for the anti-ADG antibody binding site. Since the number of sites is limited, as more sites are occupied by ADG from the sample, fewer sites are left to bind ADG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADG concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Androgen ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 92-108% | |
Linearity | Diluent Ratio | Range % |
1:2 | 99-114 | |
1:4 | 89-106 | |
1:8 | 97-109 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ADG and analogues was observed. | |
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Summary of the Assay Procedure for Mouse Androgen ELISA kit
Citations of Mouse Androgen ELISA kit
E03A0019 has been referenced in the below publications:
Mechanism of cadmium poisoning on testicular injury in mice.
