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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Angiotensin 1 ELISA kit (E03A0201)

E03A0201 Mouse Angiotensin 1 ELISA kit

Mouse Angiotensin 1 ELISA kit is suitable for the detection of samples from mouse species. Angiotensin 1 can also be called Angiotensin 1-10, Angiotensin I, Ang I, ANG1, ANG 1, ANG-1.

Products

Specifications of Mouse Angiotensin 1 ELISA kit

Product Information

Cat. No.

E03A0201

Product Name

Mouse Angiotensin 1 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

5-10 ng/mL

Sensitivity

0.1 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C



Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

0.5 ng/mL

1 vial

STANDARD C (0.5mL)

1.0 ng/mL

1 vial

STANDARD D (0.5mL)

2.5 ng/mL

1 vial

STANDARD E (0.5mL)

5.0 ng/mL

1 vial

STANDARD F (0.5mL)

10 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ANG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ANG1 antibody and an ANG1-HRP conjugate. The assay sample and buffer are incubated together with ANG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANG1 concentration since ANG1 from samples and ANG1-HRP conjugate compete for the anti-ANG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ANG1 from the sample, fewer sites are left to bind ANG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG1 concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Angiotensin 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

97-109%

Linearity

Diluent Ratio

Range %

1:2

93-106

1:4

90-109

1:8

94-101

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ANG1 and analogues was observed.



BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Angiotensin 1 ELISA kit

Summary of the Assay Procedure for Mouse Angiotensin 1 ELISA kit

Citations of Mouse Angiotensin 1 ELISA kit

E03A0201 has been referenced in the below publications:

Neutrophil-Endothelial Interactions Mediate Angiopoietin-2-Associated Pulmonary Endothelial Cell Dysfunction in Indirect Acute Lung Injury in Mice.

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