E03A0202 Mouse Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA kit
Mouse Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA kit is suitable for the detection of samples from mouse species. Mullerian Inhibiting Substance/Anti Mullerian Hormone can also be called MIS/AMH, MIS_HUMAN, Anti muellerian hormone, MIF, Muellerian inhibiting factor, Mullerian inhibiting substance, Anti-Muellerian hormone.
Product Information | |
Cat. No. | E03A0202 |
Product Name | Mouse Mullerian Inhibiting Substance/Anti Mullerian Hormone ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
MIS/AMH ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MIS/AMH antibody and an MIS/AMH-HRP conjugate. The assay sample and buffer are incubated together with MIS/AMH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIS/AMH concentration since MIS/AMH from samples and MIS/AMH-HRP conjugate compete for the anti-MIS/AMH antibody binding site. Since the number of sites is limited, as more sites are occupied by MIS/AMH from the sample, fewer sites are left to bind MIS/AMH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIS/AMH concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 90-109% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-111 | |
1:4 | 91-105 | |
1:8 | 93-107 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between MIS/AMH and analogues was observed. | |
E03A0202 has been referenced in the below publications:
Improving Effect of Sex Hormones on Ovarian Function in Mice with Ovarian Injury.
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