E05E0023 Guinea Pig E2 Estradiol ELISA kit
Guinea Pig E2 estradiol ELISA kit is suitable for the detection of samples from guinea Pig species. E2 estradiol can also be called 17B-Estradiol, E2, Oestradiol, Beta-Estradiol.
Specifications of Guinea Pig E2 Estradiol ELISA kit
Product Information | |
Cat. No. | E05E0023 |
Product Name | Guinea Pig E2 estradiol ELISA kit |
Species | Guinea Pig |
Product Size | 48 Tests / 96 Tests |
Concentration | 250-5000pg/mL |
Sensitivity | 1.0pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 250 pg/mL | 1 vial |
STANDARD C (0.5mL) | 500 pg/mL | 1 vial |
STANDARD D (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD E (0.5mL) | 2500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 5000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
E2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-E2 antibody and an E2-HRP conjugate. The assay sample and buffer are incubated together with E2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E2 concentration since E2 from samples and E2-HRP conjugate compete for the anti-E2 antibody binding site. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind E2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Guinea Pig E2 Estradiol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 88-115.79% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-112 | |
1:4 | 88-105 | |
1:8 | 83 -103 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between E2 and analogues was observed. | |
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Summary of the Assay Procedure for Guinea Pig E2 Estradiol ELISA kit
Citations of Guinea Pig E2 Estradiol ELISA kit
E05E0023 has been referenced in the below publications:
Conjugated linoleic acid mitigates testosterone-related changes in body composition in male guinea pigs.
