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BlueGene Biotech Guinea Pig Eosinophil Cationic Protein ELISA kit (E05E0125)

  • Immunology

E05E0125 Guinea Pig Eosinophil Cationic Protein ELISA kit

Guinea Pig Eosinophil cationic protein ELISA kit is suitable for the detection of samples from guinea Pig species. Eosinophil cationic protein can also be called ribonuclease 3, Rnase-A3, RNASE3, ECP, RNS3, RAF1, ribonuclease A family member 3.

Products

Specifications of Guinea Pig Eosinophil Cationic Protein ELISA kit

Product Information

Cat. No.

E05E0125

Product Name

Guinea Pig Eosinophil cationic protein ELISA kit

Species

Guinea Pig

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

ECP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ECP antibody and an ECP-HRP conjugate. The assay sample and buffer are incubated together with ECP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ECP concentration since ECP from samples and ECP-HRP conjugate compete for the anti-ECP antibody binding site. Since the number of sites is limited, as more sites are occupied by ECP from the sample, fewer sites are left to bind ECP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ECP concentration in each sample is interpolated from this standard curve.


Quality Control on Guinea Pig Eosinophil Cationic Protein ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

88-116.97%

Linearity

Diluent Ratio

Range %

1:2

98-1115

1:4

94-108

1:8

90-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between ECP and analogues was observed.


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Summary of the Assay Procedure for Guinea Pig Eosinophil Cationic Protein ELISA kit

Summary of the Assay Procedure for Guinea Pig Eosinophil Cationic Protein ELISA kit

Citations of Guinea Pig Eosinophil Cationic Protein ELISA kit

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