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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Goat Calcium Binding Protein D9K ELISA kit (E06C2509)

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E06C2509 Goat Calcium Binding Protein D9K ELISA kit

Goat Calcium Binding Protein D9K ELISA kit is suitable for the detection of samples from goat species. Calcium Binding Protein D9K can also be called CABPD9k, Calbindin-1 or Vitamin D-Dependent Calcium Binding Protein.

Products

Specifications of Goat Calcium Binding Protein D9K ELISA kit

Product Information

Cat. No.

E06C2509

Product Name

Goat Calcium Binding Protein D9K ELISA kit

Species

Goat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/mL

Sensitivity

1.0 pg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CABPD9k ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CABPD9k antibody and a CABPD9k-HRP conjugate. The assay sample and buffer are incubated together with CABPD9k-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CABPD9k concentration since CABPD9k from samples and CABPD9k-HRP conjugate compete for the anti- CABPD9k antibody binding site. Since the number of sites is limited, as more sites are occupied by CABPD9k from the sample, fewer sites are left to bind CABPD9k-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CABPD9k concentration in each sample is interpolated from this standard curve.


Quality Control on Goat Calcium Binding Protein D9K ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-116.86%

Linearity

Diluent Ratio

Range %

1:2

92-111

1:4

88-106

1:8

85-102

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CABPD9k and analogues was observed.



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Summary of the Assay Procedure for Goat Calcium Binding Protein D9K ELISA kit

Summary of the Assay Procedure for Goat Calcium Binding Protein D9K ELISA kit

Citations of Goat Calcium Binding Protein D9K ELISA kit

E06C2509 has been referenced in the below publications:

Synergy effects of lowering dietary cation-anion difference with VD and Mg supplementation on hypocalcemia prevention.

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