E07C0086 Porcine Catalase ELISA kit
Porcine Catalase ELISA kit is suitable for detecting samples from porcine species. Catalase can also be called Cas1, CAT, Cs1.
Specifications of Porcine Catalase ELISA kit
Product Information | |
Cat. No. | E07C0086 |
Product Name | Porcine Catalase ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 1-25 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CAT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CAT antibody and an CAT-HRP conjugate. The assay sample and buffer are incubated together with CAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAT concentration since CAT from samples and CAT-HRP conjugate compete for the anti-CAT antibody binding site. Since the number of sites is limited, as more sites are occupied by CAT from the sample, fewer sites are left to bind CAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAT concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Catalase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-106% | |
Linearity | Diluent Ratio | Range % |
1:2 | 90-102 | |
1:4 | 86-114 | |
1:8 | 94-105 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CAT and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Porcine Catalase ELISA kit
Citations of Porcine Catalase ELISA kit
E07C0086 has been referenced in the below publications:
Chitosan oligosaccharide affects antioxidant defense capacity and placental amino acids transport of sows.
